Hannah Ambrose was the daughter of James Ambrose and Mary Lyon.

Hannah was born around 1755.

She was christened on Sunday 13 July 1755 at Rainford, Lancashire.

Hannah married William Nicholson on Tuesday 14 July 1778 in Wigan, Lancashire.

She had the following children from her marriage to William Nicholson:

- Mary Nicholson	born around 1778.
- Betty Nicholson	born around 1780.
- John Nicholson	born around 1782.
- Ann Nicholson	born around 1783.
- William Nicholson	born around 1785 at Winstanley, Lancashire.
- Hannah Nicholson	born around 1787.
- Peter Nicholson	born around 1790.
- James Nicholson	born around 1792.
- John Nicholson	born around 1794.
- Thomas Nicholson	born around 1796 in Winstanley, Wigan, Lancashire.

Details of Hannah's death are not known.