James Ambrose was the son of Ellis Ambrose and Mary Shuttleworth.

James was born around 1719.

He was christened at Ormskirk Parish Church on 6th April 1719.

James married Mary Lyon in 1748 in Wigan, Lancashire on 19th November 1748.

He had the following children from his marriage to Mary Lyon:

- Margaret Ambrose	born around 1749.
- Ellis Ambrose	born around 1750.
- Mary Ambrose	born around 1753.
- Hannah Ambrose	born around 1755.
- Martha Ambrose	born around 1757.
- John Ambrose	born around 1759.
- Jane Ambrose	born around 1761.
- Alice Ambrose	born around 1764.
- Ann Ambrose	born around 1766.

Details of James's occupation are not known.

Details of James's death are not known.