Details of Ann's birth are not known.

Ann married Ellis Ambrose on Wednesday 30 October 1776 in Wigan, Lancashire.

She had the following children from her marriage to Ellis Ambrose:

- Mary Ambrose	born around 1777.
- James Ambrose	born around 1778.
- Ellen Ambrose	born around 1780.
- Ellis Ambrose	born around 1782.
- Charlotte Ambrose	born around 1786.
- Ann Ambrose	born around 1789.
- John Ambrose	born around 1792.
- William Ambrose	born around 1794.
- Charlotte Ambrose	born around 1801 at Upholland, Lancashire.
- Mary Ambrose	born around 1802.